GFI1B (Homo sapiens)
C2H2 ZF

TF Information

Pfam ID Interpro ID Gene ID CIS-BP ID Sequence source Animal TF db
PF00096 (zf-C2H2) IPR007087 ENSG00000165702 T095034_2.00 Ensembl (2018-Dec-8) Link out
NCBI Gene Info:
This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Directly determined binding motifs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
GFI1B
M00239_2.00
Homo sapiens
NNAAATCWSDN

NHSWGATTTNN
PBM
Barrera et al.(2016)
GFI1B_A204T
(Direct) (Direct)
GFI1B
M00240_2.00
Homo sapiens
NNAAATCWSDN

NHSWGATTTNN
PBM
Barrera et al.(2016)
GFI1B_REF
(Direct) (Direct)
GFI1B
M04523_2.00
Homo sapiens
NMAATCASDGCNNBBCACTNN

NNAGTGVVNNGCHSTGATTKN
SELEX
Yin et al.(2017)
GFI1B_eDBD_HT-SELEX
(Direct) (Direct)
GFI1B
M04524_2.00
Homo sapiens
NMAATCASWGCDNNBCACTHN

NDAGTGVNNHGCWSTGATTKN
SELEX
Yin et al.(2017)
GFI1B_eDBD_Methyl-HT-SELEX
(Direct) (Direct)
GFI1B
M08909_2.00
Homo sapiens
MAATCWSDGY

RCHSWGATTK
Misc
Kulakovskiy et al.(2013)
GFI1B_HUMAN.H11MO.0.A
(Direct) (Direct)
GFI1B
M10243_2.00
Homo sapiens Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
V$GFI1B_01
(Direct) (Direct)
GFI1B
M10244_2.00
Homo sapiens Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
V$GFI1B_Q6
(Direct) (Direct)

Motifs from related TFs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
sens
M03666_2.00
Drosophila melanogaster
TGCWSTGATTTATY

RATAAATCASWGCA
SELEX
Nitta et al.(2015)
sens_1
0.789 0.859
sens
M06056_2.00
Drosophila melanogaster
AAATCACDGC

GCHGTGATTT
B1H
Zhu et al.(2011)
sens_SANGER_10_FBgn0002573
0.789 0.859
sens
M06057_2.00
Drosophila melanogaster
NNNNAAATCACRGCH

DGCYGTGATTTNNNN
B1H
Zhu et al.(2011)
sens_SOLEXA_5_FBgn0002573
0.789 0.859
Gfi1b
M08098_2.00
Mus musculus
MAATCWCWGCH

DGCWGWGATTK
ChIP-seq
Mathelier et al.(2014)
MA0483.1
0.825 0.993
Gfi1b
M08977_2.00
Mus musculus
MAAYCACWGH

DCWGWGRTTK
Misc
Kulakovskiy et al.(2013)
GFI1B_MOUSE.H11MO.0.A
0.825 0.993
Gfi1b
M09514_2.00
Mus musculus
MAATCWSWGC

GCWSWGATTK
Misc
Heinz et al.(2010)
HPC7-Gfi1b_GSE22178
0.825 0.993
sens-2
M06165_2.00
Drosophila melanogaster
RYAAATCWSDGCAYNC

GNRTGCHSWGATTTRY
B1H
Zhu et al.(2011)
sens-2_SANGER_2.5_FBgn0051632
0.782 0.833
GFI1
M00238_2.00
Homo sapiens
NWAWMNNNN

NNNNKWTWN
PBM
Barrera et al.(2016)
GFI1_REF
0.781 0.877
GFI1
M04507_2.00
Homo sapiens
BMAATCACDGCNHBBCACTMN

NKAGTGVVDNGCHGTGATTKV
SELEX
Yin et al.(2017)
GFI1_eDBD_HT-SELEX
0.781 0.877
Gfi1
M02668_2.00
Rattus norvegicus
NMAATCWVHS

SDBWGATTKN
SELEX
Mathelier et al.(2014)
MA0038.1
0.781 0.877
GFI1
M08905_2.00
Homo sapiens
MAATCWSWGY

RCWSWGATTK
Misc
Kulakovskiy et al.(2013)
GFI1_HUMAN.H11MO.0.C
0.781 0.877
Gfi1
M08984_2.00
Mus musculus
MAATCWSWGY

RCWSWGATTK
Misc
Kulakovskiy et al.(2013)
GFI1_MOUSE.H11MO.0.C
0.781 0.877
GFI1
M10235_2.00
Homo sapiens
NNNNNNNRCHSWGATTTDNNBNNN

NNNVNNHAAATCWSDGYNNNNNNN
Transfac
Matys et al.(2006)
V$GFI1_01
0.781 0.877
GFI1
M10236_2.00
Homo sapiens Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
V$GFI1_Q6_01
0.781 0.877
GFI1
M04508_2.00
Homo sapiens
NNAGTGVVNHGCWSTGATTKV

BMAATCASWGCDNBBCACTNN
SELEX
Yin et al.(2017)
GFI1_eDBD_Methyl-HT-SELEX
0.781 0.877
GFI1
M00236_2.00
Homo sapiens
NAATMNNNN

NNNNKATTN
PBM
Barrera et al.(2016)
GFI1_L400F
0.779 0.870
For this family, TFs with SR scores > 0.755 will likely have a similar motif

Experimental Constructs

Motif ID Domain From To Sequence
M00239_2.00 C2H2 ZF 6 29
M00239_2.00 C2H2 ZF 35 57
M00239_2.00 C2H2 ZF 63 85
M00239_2.00 C2H2 ZF 91 113
M00239_2.00 C2H2 ZF 119 141
M00239_2.00 C2H2 ZF 147 170
M00240_2.00 C2H2 ZF 6 29
M00240_2.00 C2H2 ZF 35 57
M00240_2.00 C2H2 ZF 63 85
M00240_2.00 C2H2 ZF 91 113
M00240_2.00 C2H2 ZF 119 141
M00240_2.00 C2H2 ZF 147 170

DNA Binding Domains

Protein ID Domain From To Sequence
ENSP00000344782 C2H2 ZF 163 186
ENSP00000344782 C2H2 ZF 192 214
ENSP00000344782 C2H2 ZF 220 242
ENSP00000344782 C2H2 ZF 248 270
ENSP00000344782 C2H2 ZF 276 298
ENSP00000344782 C2H2 ZF 304 327
ENSP00000361195 C2H2 ZF 163 186
ENSP00000361195 C2H2 ZF 192 214
ENSP00000361195 C2H2 ZF 220 242
ENSP00000361195 C2H2 ZF 248 270
ENSP00000361195 C2H2 ZF 276 298
ENSP00000361195 C2H2 ZF 304 327
ENSP00000361196 C2H2 ZF 174 196
ENSP00000361196 C2H2 ZF 202 224
ENSP00000361196 C2H2 ZF 230 252
ENSP00000361196 C2H2 ZF 258 281

Links

Other C2H2 ZF family TFs
Other Homo sapiens TFs

196 Related TFs

Name Species Gene ID Motif Evidence SR
Score
Action
ACOC_0000424101 Angiostrongylus costaricensis ACOC_0000424101 I 0.000
ANCCEY_07004 Ancylostoma ceylanicum ANCCEY_07004 I 0.000
ASU_12730 Ascaris suum ASU_12730 N 0.000
CBG03150 Caenorhabditis briggsae CBG03150 I 0.000
CBN06159 Caenorhabditis brenneri CBN06159 I 0.000
CBN13709 Caenorhabditis brenneri CBN13709 I 0.000
CJA05669 Caenorhabditis japonica CJA05669 I 0.000
EVEC_0000289301 Enterobius vermicularis EVEC_0000289301 N 0.000
L596_g5847 Steinernema carpocapsae L596_g5847 N 0.000
L889_g20553 Steinernema feltiae L889_g20553 N 0.000
L892_g17246 Steinernema scapterisci L892_g17246 N 0.000
L893_g9725 Steinernema glaseri L893_g9725 N 0.000
NECAME_12135 Necator americanus NECAME_12135 I 0.000
PEQ_0000995301 Parascaris equorum PEQ_0000995301 N 0.000
TCNE_0001756201 Toxocara canis TCNE_0001756201 N 0.000
TELCIR_00741 Teladorsagia circumcincta TELCIR_00741 I 0.000