SMAD4 (Homo sapiens)

TF Information

Pfam ID Interpro ID Gene ID CIS-BP ID Sequence source Animal TF db
PF03165 (MH1) IPR003619 ENSG00000141646 T324625_2.00 Ensembl (2018-Dec-8) Link out
NCBI Gene Info:
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]

Directly determined binding motifs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Homo sapiens

Kulakovskiy et al.(2013)
(Direct) (Direct)
Homo sapiens

Heinz et al.(2010)
(Direct) (Direct)
Homo sapiens Transfac license required
Transfac license required
Matys et al.(2006)
(Direct) (Direct)
Homo sapiens Transfac license required
Transfac license required
Matys et al.(2006)
(Direct) (Direct)
Homo sapiens Transfac license required
Transfac license required
Matys et al.(2006)
(Direct) (Direct)

Motifs from related TFs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Mus musculus

Isakova et al.(2017)
1.000 1.000
Mus musculus

Kulakovskiy et al.(2013)
1.000 1.000
Drosophila melanogaster

Zhu et al.(2011)
0.982 0.982
Drosophila melanogaster

Kulakovskiy et al.(2009)
0.982 0.982
Caenorhabditis elegans

Weirauch et al.(2014)
0.727 0.727
For this family, TFs with SR scores > 0.700 will likely have a similar motif

DNA Binding Domains

Protein ID Domain From To Sequence
ENSP00000341551 SMAD 36 137
ENSP00000381452 SMAD 36 137
ENSP00000464772 SMAD 36 137
ENSP00000464901 SMAD 36 137
ENSP00000465874 SMAD 36 120
ENSP00000465878 SMAD 36 126
ENSP00000466934 SMAD 36 90
ENSP00000466941 SMAD 36 137
ENSP00000468611 SMAD 36 137


Other SMAD family TFs
Other Homo sapiens TFs

239 Related TFs

Name Species Gene ID Motif Evidence SR
nAv.1.0.1.g00430 Acanthocheilonema viteae nAv.1.0.1.g00430 I 0.775
ACYPI008450 Acyrthosiphon pisum ACYPI008450 I 0.892
ACYPI005550 Acyrthosiphon pisum ACYPI005550 I 0.882
ACYPI003759 Acyrthosiphon pisum ACYPI003759 I 0.902
ACYPI000365 Acyrthosiphon pisum ACYPI000365 I 0.951
AAEL013896 Aedes aegypti AAEL013896 I 0.971
ENSAMEG00000002899 Ailuropoda melanoleuca ENSAMEG00000002899 I 1.000
Alligator_sinensis_CCG008910.1 Alligator sinensis Alligator_sinensis_CCG008910.1 I 1.000
Alligator_sinensis_CCG001823.1 Alligator sinensis Alligator_sinensis_CCG001823.1 I 0.882
Aqu1.215834 Amphimedon queenslandica Aqu1.215834 I 0.873
Aqu1.215833 Amphimedon queenslandica Aqu1.215833 I 0.873
Aqu1.229767 Amphimedon queenslandica Aqu1.229767 I 0.745
ENSAPLG00000003924 Anas platyrhynchos ENSAPLG00000003924 I 1.000
ANCCAN_20978 Ancylostoma caninum ANCCAN_20978 I 0.706
ANCDUO_13021 Ancylostoma duodenale ANCDUO_13021 I 0.727
ACAC_0000977101 Angiostrongylus cantonensis ACAC_0000977101 I 0.727
ASIM_0001604001 Anisakis simplex ASIM_0001604001 I 0.804
SMAD4 Anolis carolinensis ENSACAG00000010186 I 1.000
AALB009485 Anopheles albimanus AALB009485 I 0.964
AARA004719 Anopheles arabiensis AARA004719 I 0.951
AATE010695 Anopheles atroparvus AATE010695 I 0.951
ACHR000067 Anopheles christyi ACHR000067 I 0.951
ACOM037505 Anopheles coluzzii ACOM037505 I 0.951
ACUA025512 Anopheles culicifacies ACUA025512 I 0.951
ADAC007516 Anopheles darlingi ADAC007516 I 0.964
ADIR002761 Anopheles dirus ADIR002761 I 0.951
AEPI002385 Anopheles epiroticus AEPI002385 I 0.951
AFAF011208 Anopheles farauti AFAF011208 I 0.951
AFUN009864 Anopheles funestus AFUN009864 I 0.951
AGAP002902 Anopheles gambiae AGAP002902 I 0.951
AMAM019778 Anopheles maculatus AMAM019778 I 0.951
AMEC018109 Anopheles melas AMEC018109 I 0.741
AMEM015931 Anopheles merus AMEM015931 I 0.951
AMIN000723 Anopheles minimus AMIN000723 I 0.951
AQUA009305 Anopheles quadriannulatus AQUA009305 I 0.951
ASTEI00079 Anopheles stephensi ASTEI00079 I 0.951
GB50071 Apis mellifera GB50071 I 0.961
Afo_R008709 Aptenodytes forsteri Afo_R008709 I 1.000
ASU_13433 Ascaris suum ASU_13433 I 0.775
ENSAMXG00000019219 Astyanax mexicanus ENSAMXG00000019219 I 0.990
ENSAMXG00000017275 Astyanax mexicanus ENSAMXG00000017275 I 0.982
ENSAMXG00000018339 Astyanax mexicanus ENSAMXG00000018339 I 0.982
ACEP19585 Atta cephalotes ACEP19585 I 0.843
BGLB014287 Biomphalaria glabrata BGLB014287 I 0.982
BGIBMGA010110 Bombyx mori BGIBMGA010110 I 0.951
ENSP00000341551-D1 Bos grunniens ENSP00000341551-D1 I 1.000
SMAD4 Bos taurus ENSBTAG00000006919 I 1.000
e_gw.452.68.1 Branchiostoma floridae e_gw.452.68.1 I 0.980
fgenesh2_pg.scaffold_230000050 Branchiostoma floridae fgenesh2_pg.scaffold_230000050 I 0.982
Bm3575 Brugia malayi Bm3575 I 0.784
BTMF_0000404601 Brugia timori BTMF_0000404601 I 0.784
Brh_R010414 Buceros rhinoceros Brh_R010414 I 1.000
CBN31444 Caenorhabditis brenneri CBN31444 I 0.764
CBG09090 Caenorhabditis briggsae CBG09090 I 0.745
sma-4 Caenorhabditis elegans WBGene00004858 D 0.727
CJA09693 Caenorhabditis japonica CJA09693 I 0.727
SMAD4 Callithrix jacchus ENSCJAG00000001910 I 1.000
Aan_R014474 Calypte anna Aan_R014474 I 0.845
Cflo_07183--XP_392838.3_APIME Camponotus floridanus Cflo_07183--XP_392838.3_APIME I 0.961
SMAD4 Canis familiaris ENSCAFG00000000164 I 1.000
CapteG179368 Capitella teleta CapteG179368 I 0.964
SMAD4 Cavia porcellus ENSCPOG00000006470 I 1.000
Cmyd_10005540 Chelonia mydas Cmyd_10005540 I 1.000
ENSCSAG00000018094 Chlorocebus sabaeus ENSCSAG00000018094 I 1.000
SMAD4 Choloepus hoffmanni ENSCHOG00000000860 I 1.000
CLEC002594 Cimex lectularius CLEC002594 I 0.951
smad4 Ciona intestinalis ENSCING00000005559 I 0.964
ENSCSAVG00000006480 Ciona savignyi ENSCSAVG00000006480 I 0.964
CGI_10018464 Crassostrea gigas CGI_10018464 I 0.911
CGI_10000594 Crassostrea gigas CGI_10000594 I 0.821
CpipJ_CPIJ002563 Culex pipiens CpipJ_CPIJ002563 I 0.961
CPIJ002563 Culex quinquefasciatus CPIJ002563 I 0.961
CGOC_0000897101 Cylicostephanus goldi CGOC_0000897101 I 0.745
KGM_12572 Danaus plexippus KGM_12572 I 0.951
SMAD4 Danio rerio ENSDARG00000012649 I 0.982
si:dkey-239n17.4 Danio rerio ENSDARG00000070428 I 0.951
smad4a Danio rerio ENSDARG00000075226 I 0.990
si:dkey-222b8.1 Danio rerio ENSDARG00000045094 I 0.982
DAPPUDRAFT_304115 Daphnia pulex DAPPUDRAFT_304115 I 0.951
SMAD4 Dasypus novemcinctus ENSDNOG00000005063 I 1.000
YQE_08737 Dendroctonus ponderosae YQE_08737 I 0.892
YQE_06624 Dendroctonus ponderosae YQE_06624 I 0.964
Smad4 Dipodomys ordii ENSDORG00000009089 I 1.000
nDi.2.2.2.g01429 Dirofilaria immitis nDi.2.2.2.g01429 I 0.775
GF22974 Drosophila ananassae FBgn0099968 I 0.982
GG11819 Drosophila erecta FBgn0104114 I 0.982
GH18642 Drosophila grimshawi FBgn0126109 I 0.982
Med Drosophila melanogaster FBgn0011655 D 0.982
GI23400 Drosophila mojavensis FBgn0146126 I 0.982
GL23637 Drosophila persimilis FBgn0161227 I 0.982
GA14643 Drosophila pseudoobscura FBgn0074670 I 0.982
GM16436 Drosophila sechellia FBgn0171351 I 0.982
GJ23092 Drosophila virilis FBgn0210194 I 0.982
GK11724 Drosophila willistoni FBgn0213735 I 0.982
GE10952 Drosophila yakuba FBgn0228782 I 0.982
SMAD4 Echinops telfairi ENSETEG00000007750 I 1.000
ECPE_0001832001 Echinostoma caproni ECPE_0001832001 I 0.794
EEL_0000707501 Elaeophora elaphi EEL_0000707501 I 0.775
EVEC_0000355201 Enterobius vermicularis EVEC_0000355201 I 0.718
SMAD4 Equus caballus ENSECAG00000017857 I 1.000
D915_03789 Fasciola hepatica D915_03789 I 0.804
SMAD4 Felis catus ENSFCAG00000005257 I 1.000
ENSGMOG00000005844 Gadus morhua ENSGMOG00000005844 I 0.980
ENSGMOG00000000297 Gadus morhua ENSGMOG00000000297 I 0.951
ENSGMOG00000013964 Gadus morhua ENSGMOG00000013964 I 1.000
ENSGACG00000016858 Gasterosteus aculeatus ENSGACG00000016858 I 0.980
smad4a Gasterosteus aculeatus ENSGACG00000007495 I 0.980
si:dkey-222b8.1 Gasterosteus aculeatus ENSGACG00000000538 I 0.982
ENSGACG00000003171 Gasterosteus aculeatus ENSGACG00000003171 I 0.902
Gfo_R016223 Geospiza fortis Gfo_R016223 I 1.000
Gfo_R011803 Geospiza fortis Gfo_R011803 I 1.000
GPLIN_000772200 Globodera pallida GPLIN_000772200 I 0.706
GPUH_0001329001 Gongylonema pulchrum GPUH_0001329001 I 0.794
SMAD4 Gorilla gorilla ENSGGOG00000013662 I 1.000
HCOI01928500 Haemonchus contortus HCOI01928500 I 0.745
HCOI02014300 Haemonchus contortus HCOI02014300 I 0.745
HHAL000394-PA Halyomorpha halys HHAL000394-PA I 0.951
HHAL000393-PA Halyomorpha halys HHAL000393-PA I 0.961
Hsal_09633--XP_392838.3_APIME Harpegnathos saltator Hsal_09633--XP_392838.3_APIME I 0.961
HMEL002203 Heliconius melpomene HMEL002203 I 0.951
HPBE_0001599301 Heligmosomoides bakeri HPBE_0001599301 I 0.702
HelroG76435 Helobdella robusta HelroG76435 I 0.784
HelroG88197 Helobdella robusta HelroG88197 I 0.891
XP_002161797.1 Hydra magnipapillata XP_002161797.1 I 0.882
ENSSTOG00000028580 Ictidomys tridecemlineatus ENSSTOG00000028580 I 1.000
ISCW018468 Ixodes scapularis ISCW018468 I 0.982
ENSLACG00000007810 Latimeria chalumnae ENSLACG00000007810 I 0.961
ENSLOCG00000012872 Lepisosteus oculatus ENSLOCG00000012872 I 0.784
ENSLOCG00000006749 Lepisosteus oculatus ENSLOCG00000006749 N 0.873
LDEC015949 Leptinotarsa decemlineata LDEC015949 I 0.964
nLs.2.1.2.g01829 Litomosoides sigmodontis nLs.2.1.2.g01829 I 0.709
nLs.2.1.2.g01457 Litomosoides sigmodontis nLs.2.1.2.g01457 I 0.775
LOAG_08923 Loa loa LOAG_08923 I 0.765
LotgiG131078 Lottia gigantea LotgiG131078 I 0.941
SMAD4 Loxodonta africana ENSLAFG00000012595 I 1.000
LCUP007928 Lucilia cuprina LCUP007928 I 0.982
LLOJ008806 Lutzomyia longipalpis LLOJ008806 I 0.982
ENSP00000381452 Macaca fascicularis ENSP00000381452 I 1.000
SMAD4 Macaca mulatta ENSMMUG00000018334 I 1.000
ENSMEUG00000001823 Macropus eugenii ENSMEUG00000001823 I 0.988
MCINX014520 Melitaea cinxia MCINX014520 I 0.951
SMAD4 Microcebus murinus ENSMICG00000017525 I 1.000
ML02191a Mnemiopsis leidyi ML02191a I 0.863
SMAD4 Monodelphis domestica ENSMODG00000010995 I 1.000
MDOA012885 Musca domestica MDOA012885 I 0.982
ENSMPUG00000008776 Mustela putorius furo ENSMPUG00000008776 I 1.000
Smad4 Mus musculus ENSMUSG00000024515 D 1.000
Myotis_brandtii_Smad4_10005924 Myotis brandtii Myotis_brandtii_Smad4_10005924 I 1.000
SMAD4 Myotis lucifugus ENSMLUG00000013944 I 1.000
NV12984 Nasonia vitripennis NV12984 I 0.961
NEMVEDRAFT_v1g18576 Nematostella vectensis NEMVEDRAFT_v1g18576 I 0.836
NEMVEDRAFT_v1g248037 Nematostella vectensis NEMVEDRAFT_v1g248037 I 0.931
ENSNLEG00000011859 Nomascus leucogenys ENSNLEG00000011859 I 1.000
SMAD4 Ochotona princeps ENSOPRG00000013601 I 1.000
Ocbimv22032358m.g Octopus bimaculoides Ocbimv22032358m.g I 0.964
OESDEN_03018 Oesophagostomum dentatum OESDEN_03018 I 0.706
nOo.2.0.1.g00181 Onchocerca ochengi nOo.2.0.1.g00181 I 0.765
WBGene00241622 Onchocerca volvulus WBGene00241622 I 0.765
Ogr06720 Ophisaurus gracilis Ogr06720 I 1.000
Ogr14199 Ophisaurus gracilis Ogr14199 I 1.000
T265_11013 Opisthorchis viverrini T265_11013 I 0.804
ENSONIG00000015035 Oreochromis niloticus ENSONIG00000015035 I 1.000
ENSONIG00000014036 Oreochromis niloticus ENSONIG00000014036 I 0.980
ENSONIG00000010360 Oreochromis niloticus ENSONIG00000010360 I 0.961
ENSONIG00000001557 Oreochromis niloticus ENSONIG00000001557 I 0.964
SMAD4 Ornithorhynchus anatinus ENSOANG00000008117 I 1.000
ENSOANG00000030959 Ornithorhynchus anatinus ENSOANG00000030959 I 1.000
SMAD4 Oryctolagus cuniculus ENSOCUG00000003929 I 1.000
smad4a Oryzias latipes ENSORLG00000005020 I 0.990
SMAD4 Oryzias latipes ENSORLG00000007703 I 1.000
SMAD4 Otolemur garnettii ENSOGAG00000012081 I 1.000
ENSOARG00000004662 Ovis aries ENSOARG00000004662 I 1.000
SMAD4 Pan troglodytes ENSPTRG00000010028 I 1.000
ENSPANG00000024703 Papio anubis ENSPANG00000024703 I 1.000
PTRK_0001020700 Parastrongyloides trichosuri PTRK_0001020700 I 0.727
PHUM002670 Pediculus humanus PHUM002670 I 0.971
ENSPSIG00000014614 Pelodiscus sinensis ENSPSIG00000014614 I 1.000
ENSPMAG00000008404 Petromyzon marinus ENSPMAG00000008404 I 0.982
ENSPMAG00000009414 Petromyzon marinus ENSPMAG00000009414 I 0.941
PPAI002168 Phlebotomus papatasi PPAI002168 I 0.982
ENSPFOG00000019807 Poecilia formosa ENSPFOG00000019807 I 1.000
ENSPFOG00000018987 Poecilia formosa ENSPFOG00000018987 I 0.990
ENSPFOG00000019867 Poecilia formosa ENSPFOG00000019867 I 0.902
Pvit_01119 Pogona vitticeps Pvit_01119 I 1.000
ENSPPYG00000009165 Pongo abelii ENSPPYG00000009165 I 1.000
SMAD4 Pteropus vampyrus ENSPVAG00000004261 I 1.000
Smad4 Rattus norvegicus ENSRNOG00000051965 I 1.000
RPRC010188 Rhodnius prolixus RPRC010188 I 0.951
Sakowv30001112m.g Saccoglossus kowalevskii Sakowv30001112m.g I 0.746
ENSSHAG00000010344 Sarcophilus harrisii ENSSHAG00000010344 I 1.000
Sjc_0030450 Schistosoma japonicum Sjc_0030450 I 0.782
Smp_033950 Schistosoma mansoni Smp_033950 I 0.745
SMTD_0002197901 Schistosoma mattheei SMTD_0002197901 I 0.745
SROB_0000384401 Schistosoma rodhaini SROB_0000384401 I 0.727
SMU15015494 Schmidtea mediterranea SMU15015494 I 0.824
SINV15516 Solenopsis invicta SINV15516 I 0.961
L596_g26024 Steinernema carpocapsae L596_g26024 I 0.727
L893_g15056 Steinernema glaseri L893_g15056 I 0.764
L892_g4495 Steinernema scapterisci L892_g4495 I 0.727
SMAR013850 Strigamia maritima SMAR013850 I 0.971
SPU_017971 Strongylocentrotus purpuratus SPU_017971 I 0.873
SPU_004287 Strongylocentrotus purpuratus SPU_004287 I 0.953
SPAL_0001099400 Strongyloides papillosus SPAL_0001099400 I 0.709
SVUK_0000938201 Strongylus vulgaris SVUK_0000938201 I 0.706
SMAD4 Sus scrofa ENSSSCG00000004524 I 1.000
gb:cr929477 Takifugu rubripes ENSTRUG00000011769 I 0.951
si:dkey-222b8.1 Takifugu rubripes ENSTRUG00000004163 I 0.982
SMAD4 Takifugu rubripes ENSTRUG00000014045 I 1.000
ENSTSYG00000009792 Tarsius syrichta ENSTSYG00000009792 I 1.000
TELCIR_06960 Teladorsagia circumcincta TELCIR_06960 I 0.745
TELCIR_19676 Teladorsagia circumcincta TELCIR_19676 I 0.706
tetur10g00400 Tetranychus urticae tetur10g00400 I 0.912
tetur10g00380 Tetranychus urticae tetur10g00380 I 0.767
si:dkey-222b8.1 Tetraodon nigroviridis ENSTNIG00000005007 I 0.902
gb:cr929477 Tetraodon nigroviridis ENSTNIG00000003193 I 0.951
SMAD4 Tetraodon nigroviridis ENSTNIG00000009050 I 1.000
TCLT_0000917301 Thelazia callipaeda TCLT_0000917301 I 0.765
TCLT_0000412001 Thelazia callipaeda TCLT_0000412001 I 0.709
TC010848 Tribolium castaneum TC010848 I 0.964
D917_09719 Trichinella nativa D917_09719 I 0.824
EFV62078 Trichinella spiralis EFV62078 I 0.824
TRE_0001987301 Trichobilharzia regenti TRE_0001987301 I 0.800
TriadG30731 Trichoplax adhaerens TriadG30731 I 0.804
TMUE_s0006012700 Trichuris muris TMUE_s0006012700 I 0.833
D918_00298 Trichuris suis D918_00298 I 0.760
TTRE_0000855901 Trichuris trichiura TTRE_0000855901 I 0.833
SMAD4 Tupaia belangeri ENSTBEG00000014105 I 1.000
SMAD4 Vicugna pacos ENSVPAG00000006757 I 1.000
WUBG_02880 Wuchereria bancrofti WUBG_02880 I 0.775
AAH84196 Xenopus laevis AAH84196 I 1.000
smad4.1.L Xenopus laevis XB-GENE-865543 I 1.000
smad4.2.S Xenopus laevis XB-GENE-5995204 I 0.982
smad4.2.L Xenopus laevis XB-GENE-6256010 I 0.982
smad4.1 Xenopus tropicalis XB-GENE-485691 I 1.000
ENSXMAG00000000346 Xiphophorus maculatus ENSXMAG00000000346 N 0.818
ENSXMAG00000015245 Xiphophorus maculatus ENSXMAG00000015245 I 0.990
ENSXMAG00000005051 Xiphophorus maculatus ENSXMAG00000005051 I 1.000
L798_13939 Zootermopsis nevadensis L798_13939 I 0.971