PIF3 (Arabidopsis thaliana)
bHLH

TF Information

Pfam ID Interpro ID Gene ID CIS-BP ID Sequence source Animal TF db
PF00010 (HLH) IPR001092 AT1G09530 T030580_2.00 Ensembl (2018-Dec-8) Link out
NCBI Gene Info:
Transcription factor interacting with photoreceptors phyA and phyB. Forms a ternary complex in vitro with G-box element of the promoters of LHY, CCA1. Acts as a negative regulator of phyB signalling. It degrades rapidly after irradiation of dark grown seedlings in a process controlled by phytochromes. Does not play a significant role in controlling light input and function of the circadian clockwork. Binds to G- and E-boxes, but not to other ACEs. Binds to anthocyanin biosynthetic genes in a light- and HY5-independent fashion. PIF3 function as a transcriptional activator can be functionally and mechanistically separated from its role in repression of PhyB mediated processes.

Directly determined binding motifs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
PIF3
M00722_2.00
Arabidopsis thaliana
NCACRTN

NAYGTGN
PBM
Chang et al.(2013)
pTH8200
(Direct) (Direct)
PIF3
M00854_2.00
Arabidopsis thaliana
CACGTGSNN

NNSCACGTG
PBM
Franco-Zorrilla et al.(2014)
PIF3
(Direct) (Direct)
PIF3
M08045_2.00
Arabidopsis thaliana
HCACGTGGVH

DBCCACGTGD
ChIP-seq
Mathelier et al.(2014)
MA0560.1
(Direct) (Direct)
PIF3
M09780_2.00
Arabidopsis thaliana
BDRRRVCACGTGRVSHCD

HGDSBYCACGTGBYYYHV
Transfac
Matys et al.(2006)
P$PIF3_01
(Direct) (Direct)
PIF3
M09781_2.00
Arabidopsis thaliana
DDDVCCACGTGGVMVNB

VNBKBCCACGTGGBHHH
Transfac
Matys et al.(2006)
P$PIF3_02
(Direct) (Direct)

Motifs from related TFs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
PK05451.1
M01120_2.00
Cannabis sativa
YCACGTGM

KCACGTGR
PBM
Lambert et al.(2019)
pTH10100
0.914 0.960
SPT
M01709_2.00
Arabidopsis thaliana
NCACGTGSN

NSCACGTGN
PBM
Weirauch et al.(2014)
pTH8111
0.877 0.840
e_gw1.209.40.1
M01756_2.00
Physcomitrella patens
NNCACGTGNN

NNCACGTGNN
PBM
Weirauch et al.(2014)
pTH8070
0.872 0.620
LRL2
M06738_2.00
Arabidopsis thaliana
CACGTGSVDMNCACG

CGTGNKHBSCACGTG
Dap-seq
OMalley et al.(2016)
bHLH69_col_a
0.872 0.580
PIF1
M01703_2.00
Arabidopsis thaliana
NNCACGTGNN

NNCACGTGNN
PBM
Weirauch et al.(2014)
pTH8733
0.868 0.860
PIF1
M08404_2.00
Arabidopsis thaliana
NCACGTGWSNYNHH

DDNRNSWCACGTGN
ChIP-chip
Mathelier et al.(2014)
MA0552.1
0.868 0.860
PIF1
M09783_2.00
Arabidopsis thaliana Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
P$PIF1_01
0.868 0.860
PIF4
M08046_2.00
Arabidopsis thaliana
CACGTGSV

BSCACGTG
ChIP-seq
Mathelier et al.(2014)
MA0561.1
0.868 0.840
PIF5
M08047_2.00
Arabidopsis thaliana
YCACRTGS

SCAYGTGR
ChIP-seq
Mathelier et al.(2014)
MA0562.1
0.855 0.820
PIF5
M09452_2.00
Arabidopsis thaliana
NNBCAYGTGV

BCACRTGVNN
Misc
Heinz et al.(2010)
Arabidopsis-PIF5ox_GSE35062
0.855 0.820
UNE10
M01705_2.00
Arabidopsis thaliana
HCACGTGM

KCACGTGD
PBM
Weirauch et al.(2014)
pTH8112
0.840 0.660
For this family, TFs with SR scores > 0.838 will likely have a similar motif

Experimental Constructs

Motif ID Domain From To Sequence
M00722_2.00 bHLH 51 100
M00854_2.00 bHLH 344 393

DNA Binding Domains

Protein ID Domain From To Sequence
AT1G09530.1 bHLH 344 393
AT1G09530.2 bHLH 344 393
AT1G09530.3 bHLH 344 393
AT1G09530.4 bHLH 344 393
AT1G09530.5 bHLH 344 393
AT1G09530.6 bHLH 344 393

Links

Other bHLH family TFs
Other Arabidopsis thaliana TFs

Invalid Input OrderBy

576 Related TFs

Name Species Gene ID Motif Evidence SR
Score
Action