CIS-BP Database: Catalog of Inferred Sequence Binding Preferences
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ENSAMXG00000008359
(
Astyanax mexicanus
)
RFX
TF Information
Pfam ID
Interpro ID
Gene ID
CIS-BP ID
Sequence source
Animal TF db
PF02257 (RFX_DNA_binding)
IPR003150
ENSAMXG00000008359
T318125_2.00
Ensembl (2018-Dec-8)
Link out
Directly determined binding motifs
Name/Motif ID
Species
Forward
Reverse
Type/Study/Study ID
SR
Score
DBD
Identity
No direct experiments
Motifs from related TFs
Name/Motif ID
Species
Forward
Reverse
Type/Study/Study ID
SR
Score
DBD
Identity
RFX5
M03458_2.00
Homo sapiens
BGTTRCYATRGYAACV
BGTTRCYATRGYAACV
SELEX
Jolma et al.(2013)
RFX5_1
0.739
0.772
RFX5
M03459_2.00
Homo sapiens
BGTTGCYATGGYAACV
BGTTRCCATRGCAACV
SELEX
Jolma et al.(2013)
RFX5_2
0.739
0.772
RFX5
M03460_2.00
Homo sapiens
BGTTGCYATGGYAACV
BGTTRCCATRGCAACV
SELEX
Jolma et al.(2013)
RFX5_3
0.739
0.772
RFX5
M05725_2.00
Homo sapiens
BGTTRCYANGBGTTGCYRN
NYRGCAACVCNTRGYAACV
SELEX
Yin et al.(2017)
RFX5_eDBD_HT-SELEX_1
0.739
0.772
RFX5
M05726_2.00
Homo sapiens
NYRVYAACSGTTRCYAN
NTRGYAACSGTTRBYRN
SELEX
Yin et al.(2017)
RFX5_eDBD_HT-SELEX_2
0.739
0.772
RFX5
M08009_2.00
Homo sapiens
KCRBYDGTTRCYRRG
CYYRGYAACHRVYGM
ChIP-seq
Gerstein et al.(2012)
GM12878_RFX5_Stanford
0.739
0.772
RFX5
M08010_2.00
Homo sapiens
DBVBYDGTTGCYRGGBVRR
YYBVCCYRGCAACHRVBVH
ChIP-seq
Gerstein et al.(2012)
H1-hESC_RFX5_Stanford
0.739
0.772
RFX5
M08011_2.00
Homo sapiens
TCVBYDGTTDCYRDG
CHYRGHAACHRVBGA
ChIP-seq
Gerstein et al.(2012)
HeLa-S3_RFX5_Stanford
0.739
0.772
RFX5
M08012_2.00
Homo sapiens
YDGTTRCYRKGBVRV
BYBVCMYRGYAACHR
ChIP-seq
Gerstein et al.(2012)
HepG2_RFX5_Stanford
0.739
0.772
RFX5
M09364_2.00
Homo sapiens
VNNVBYDGTTGCYRKGNVRV
BYBNCMYRGCAACHRVBNNB
Misc
Kulakovskiy et al.(2013)
RFX5_HUMAN.H11MO.0.A
0.739
0.772
RFX5
M09629_2.00
Homo sapiens
NDGTTRCYADGV
BCHTRGYAACHN
Misc
Heinz et al.(2010)
GM12878-Rfx5_GSE31477
0.739
0.772
RFX5
M11249_2.00
Homo sapiens
Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
V$RFX5_03
0.739
0.772
RFX5
M11250_2.00
Homo sapiens
Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
V$RFX5_05
0.739
0.772
RFX5
M05727_2.00
Homo sapiens
BGTYRYYRNGBGTTGYYRN
NYRRCAACVCNYRRYRACV
SELEX
Yin et al.(2017)
RFX5_eDBD_Methyl-HT-SELEX_1
0.739
0.772
RFX5
M05728_2.00
Homo sapiens
NTRGYAASSGTYRBYRN
NYRVYRACSSTTRCYAN
SELEX
Yin et al.(2017)
RFX5_eDBD_Methyl-HT-SELEX_2
0.739
0.772
Rfx7
M00823_2.00
Mus musculus
GTYRCYRNSB
VSNYRGYRAC
PBM
Weirauch et al.(2013)
pTH3516
0.712
0.620
Rfx7
M00187_2.00
Mus musculus
BGTHDYYRNN
NNYRRHDACV
PBM
Badis et al.(2009)
Rfxdc2_3516
0.712
0.620
RFX7
M05729_2.00
Homo sapiens
BGTTRCYRY
RYRGYAACV
SELEX
Yin et al.(2017)
RFX7_eDBD_HT-SELEX
0.712
0.620
RFX7
M05730_2.00
Homo sapiens
BGTTGCYRB
VYRGCAACV
SELEX
Yin et al.(2017)
RFX7_eDBD_Methyl-HT-SELEX
0.712
0.620
For this family, TFs with SR scores >
0.650
will likely have a similar motif
DNA Binding Domains
Protein ID
Domain
From
To
Sequence
ENSAMXP00000008591
RFX
81
163
QLHTCNWIRSHLEEHADTCLPKQDVYETYRKHCENLQQRPLSAANFGKIIRDIFPNIKARRLGGRGQSKTLHTYCYSGIRRKT
Links
Other
RFX
family TFs
Other
Astyanax mexicanus
TFs
231 Related TFs
Name
Species
Gene ID
Motif Evidence
SR
Score
Action
ENSLOCG00000008459
Lepisosteus oculatus
ENSLOCG00000008459
I
0.793
rfx5
Danio rerio
ENSDARG00000063258
I
0.792
ENSONIG00000003955
Oreochromis niloticus
ENSONIG00000003955
I
0.790
ENSPFOG00000010592
Poecilia formosa
ENSPFOG00000010592
I
0.789
rfx5
Tetraodon nigroviridis
ENSTNIG00000005388
I
0.789
ENSTRUG00000000177
Takifugu rubripes
ENSTRUG00000000177
I
0.789
ENSXMAG00000017295
Xiphophorus maculatus
ENSXMAG00000017295
I
0.789
ENSORLG00000001269
Oryzias latipes
ENSORLG00000001269
I
0.776
RFX5
Gallus gallus
ENSGALG00000000836
I
0.763
rfx5
Xenopus tropicalis
XB-GENE-5942579
I
0.763
ENSFALG00000010929
Ficedula albicollis
ENSFALG00000010929
I
0.760
Gfo_R003263
Geospiza fortis
Gfo_R003263
I
0.760
ENSMGAG00000001126
Meleagris gallopavo
ENSMGAG00000001126
I
0.758
Afo_R007667
Aptenodytes forsteri
Afo_R007667
I
0.756
RFX5
Procavia capensis
ENSPCAG00000011056
I
0.755
Alligator_sinensis_CCG020683.1
Alligator sinensis
Alligator_sinensis_CCG020683.1
I
0.755
ENSAMEG00000000695
Ailuropoda melanoleuca
ENSAMEG00000000695
I
0.750
RFX5
Myotis lucifugus
ENSMLUG00000015218
I
0.750
Aan_R010941
Calypte anna
Aan_R010941
I
0.750
Myotis_brandtii_RFX5_10008035
Myotis brandtii
Myotis_brandtii_RFX5_10008035
I
0.750
Pvit_04986
Pogona vitticeps
Pvit_04986
I
0.749
rfx5.S
Xenopus laevis
XB-GENE-6079297
I
0.749
ENSACAG00000029645
Anolis carolinensis
ENSACAG00000029645
I
0.748
ENSPSIG00000018073
Pelodiscus sinensis
ENSPSIG00000018073
I
0.748
ENSSTOG00000013246
Ictidomys tridecemlineatus
ENSSTOG00000013246
I
0.741
Ogr13052
Ophisaurus gracilis
Ogr13052
I
0.741
ENSMEUG00000011101
Macropus eugenii
ENSMEUG00000011101
I
0.740
ENSSHAG00000014366
Sarcophilus harrisii
ENSSHAG00000014366
I
0.740
RFX5
Bos taurus
ENSBTAG00000017580
I
0.739
RFX5
Canis familiaris
ENSCAFG00000012661
I
0.739
RFX5
Callithrix jacchus
ENSCJAG00000007482
I
0.739
ENSCPOG00000010643
Cavia porcellus
ENSCPOG00000010643
I
0.739
ENSCSAG00000001799
Chlorocebus sabaeus
ENSCSAG00000001799
I
0.739
ENSDORG00000015967
Dipodomys ordii
ENSDORG00000015967
I
0.739
RFX5
Equus caballus
ENSECAG00000024774
I
0.739
RFX5
Echinops telfairi
ENSETEG00000013503
I
0.739
RFX5
Felis catus
ENSFCAG00000005568
I
0.739
RFX5
Homo sapiens
ENSG00000143390
D
0.739
RFX5
Gorilla gorilla
ENSGGOG00000016795
I
0.739
RFX5
Loxodonta africana
ENSLAFG00000030906
I
0.739
ENSMMUG00000013172
Macaca mulatta
ENSMMUG00000013172
I
0.739
ENSMPUG00000003735
Mustela putorius furo
ENSMPUG00000003735
I
0.739
Rfx5
Mus musculus
ENSMUSG00000005774
I
0.739
ENSNLEG00000010170
Nomascus leucogenys
ENSNLEG00000010170
I
0.739
ENSOARG00000020987
Ovis aries
ENSOARG00000020987
I
0.739
ENSOCUG00000011564
Oryctolagus cuniculus
ENSOCUG00000011564
I
0.739
RFX5
Otolemur garnettii
ENSOGAG00000008120
I
0.739
ENSPANG00000005712
Papio anubis
ENSPANG00000005712
I
0.739
ENSPPYG00000000867
Pongo abelii
ENSPPYG00000000867
I
0.739
RFX5
Pan troglodytes
ENSPTRG00000001286
I
0.739
RFX5
Pteropus vampyrus
ENSPVAG00000011680
I
0.739
Rfx5
Rattus norvegicus
ENSRNOG00000021012
I
0.739
RFX5
Sorex araneus
ENSSARG00000000750
I
0.739
RFX5
Sus scrofa
ENSSSCG00000006625
I
0.739
RFX5
Tupaia belangeri
ENSTBEG00000006419
I
0.739
RFX5
Tursiops truncatus
ENSTTRG00000007023
I
0.739
ENSP00000389130-D1
Bos grunniens
ENSP00000389130-D1
I
0.739
ENSP00000389130
Macaca fascicularis
ENSP00000389130
I
0.739
RFX5
Choloepus hoffmanni
ENSCHOG00000012900
I
0.735
RFX5
Erinaceus europaeus
ENSEEUG00000010785
I
0.735
ENSLOCG00000013505
Lepisosteus oculatus
ENSLOCG00000013505
I
0.717
ENSAMXG00000014807
Astyanax mexicanus
ENSAMXG00000014807
I
0.716
ENSAMXG00000020093
Astyanax mexicanus
ENSAMXG00000020093
I
0.716
RFX7
Danio rerio
ENSDARG00000103691
I
0.716
RFX7
Gasterosteus aculeatus
ENSGACG00000006090
I
0.716
ENSGMOG00000004164
Gadus morhua
ENSGMOG00000004164
I
0.716
ENSONIG00000008334
Oreochromis niloticus
ENSONIG00000008334
I
0.716
RFX7
Oryzias latipes
ENSORLG00000005342
I
0.716
ENSXMAG00000006676
Xiphophorus maculatus
ENSXMAG00000006676
I
0.716
ENSPMAG00000005066
Petromyzon marinus
ENSPMAG00000005066
I
0.715
RFX7
Anolis carolinensis
ENSACAG00000008482
I
0.712
ENSAMEG00000018368
Ailuropoda melanoleuca
ENSAMEG00000018368
I
0.712
ENSAPLG00000005911
Anas platyrhynchos
ENSAPLG00000005911
I
0.712
RFX7
Bos taurus
ENSBTAG00000000565
I
0.712
RFX7
Canis familiaris
ENSCAFG00000016029
I
0.712
RFX7
Choloepus hoffmanni
ENSCHOG00000003451
I
0.712
ENSCPOG00000005366
Cavia porcellus
ENSCPOG00000005366
I
0.712
ENSCSAG00000011819
Chlorocebus sabaeus
ENSCSAG00000011819
I
0.712
RFX7
Dasypus novemcinctus
ENSDNOG00000044983
I
0.712
ENSFALG00000012087
Ficedula albicollis
ENSFALG00000012087
I
0.712
RFX7
Homo sapiens
ENSG00000181827
D
0.712
ENSGMOG00000002477
Gadus morhua
ENSGMOG00000002477
I
0.712
ENSLACG00000015878
Latimeria chalumnae
ENSLACG00000015878
I
0.712
RFX7
Loxodonta africana
ENSLAFG00000018745
I
0.712
RFX7
Meleagris gallopavo
ENSMGAG00000005745
I
0.712
RFX7
Monodelphis domestica
ENSMODG00000008136
I
0.712
ENSMPUG00000002204
Mustela putorius furo
ENSMPUG00000002204
I
0.712
Rfx7
Mus musculus
ENSMUSG00000037674
D
0.712
ENSNLEG00000006658
Nomascus leucogenys
ENSNLEG00000006658
I
0.712
RFX7
Ornithorhynchus anatinus
ENSOANG00000008526
I
0.712
RFX7
Oryctolagus cuniculus
ENSOCUG00000013678
I
0.712
ENSPANG00000017297
Papio anubis
ENSPANG00000017297
I
0.712
RFX7
Procavia capensis
ENSPCAG00000004974
I
0.712
ENSPPYG00000006502
Pongo abelii
ENSPPYG00000006502
I
0.712
ENSPSIG00000014731
Pelodiscus sinensis
ENSPSIG00000014731
I
0.712
RFX7
Pan troglodytes
ENSPTRG00000007104
I
0.712
Rfx7
Rattus norvegicus
ENSRNOG00000060367
I
0.712
ENSSHAG00000003875
Sarcophilus harrisii
ENSSHAG00000003875
I
0.712
ENSTSYG00000002441
Tarsius syrichta
ENSTSYG00000002441
I
0.712
RFX7
Tursiops truncatus
ENSTTRG00000010051
I
0.712
RFX7
Vicugna pacos
ENSVPAG00000012402
I
0.712
rfx7
Danio rerio
ENSDARG00000077237
I
0.711
rfx7
Xenopus tropicalis
XB-GENE-1008447
I
0.711
RFX7
Pteropus vampyrus
ENSPVAG00000005381
I
0.710
RFX7
Tetraodon nigroviridis
ENSTNIG00000013706
I
0.710
RFX7
Takifugu rubripes
ENSTRUG00000008008
I
0.710
ENSOARG00000020885
Ovis aries
ENSOARG00000020885
I
0.709
RFX7
Otolemur garnettii
ENSOGAG00000031795
I
0.709
Ocbimv22017482m.g
Octopus bimaculoides
Ocbimv22017482m.g
I
0.708
RFX7
Equus caballus
ENSECAG00000002379
I
0.707
rfx7
Gasterosteus aculeatus
ENSGACG00000015838
I
0.707
RFX7
Myotis lucifugus
ENSMLUG00000028676
I
0.707
RFX7
Taeniopygia guttata
ENSTGUG00000006356
I
0.707
Aan_R011989
Calypte anna
Aan_R011989
I
0.707
Afo_R011605
Aptenodytes forsteri
Afo_R011605
I
0.707
ENSP00000397644-D1
Bos grunniens
ENSP00000397644-D1
I
0.707
Gfo_R004305
Geospiza fortis
Gfo_R004305
I
0.707
csin102559
Clonorchis sinensis
csin102559
I
0.707
T265_11740
Opisthorchis viverrini
T265_11740
I
0.707
RFX7
Felis catus
ENSFCAG00000029408
I
0.706
RFX7
Macaca mulatta
ENSMMUG00000017801
I
0.706
RFX7
Sorex araneus
ENSSARG00000010801
I
0.706
ENSMMUP00000033587
Macaca fascicularis
ENSMMUP00000033587
I
0.706
SPU_027998
Strongylocentrotus purpuratus
SPU_027998
I
0.705
Myotis_brandtii_RFX7_10006263
Myotis brandtii
Myotis_brandtii_RFX7_10006263
I
0.705
SMU15027042
Schmidtea mediterranea
SMU15027042
I
0.705
Pvit_17399
Pogona vitticeps
Pvit_17399
I
0.704
rfx7
Oryzias latipes
ENSORLG00000008583
I
0.703
e_gw.237.152.1
Branchiostoma floridae
e_gw.237.152.1
I
0.703
RFX7
Erinaceus europaeus
ENSEEUG00000013718
I
0.699
Alligator_sinensis_CCG008945.1
Alligator sinensis
Alligator_sinensis_CCG008945.1
I
0.699
Brh_R008414
Buceros rhinoceros
Brh_R008414
I
0.699
ECANG7_06078
Echinococcus canadensis
ECANG7_06078
I
0.699
EmuJ_001055400
Echinococcus multilocularis
EmuJ_001055400
I
0.699
ENSONIG00000005780
Oreochromis niloticus
ENSONIG00000005780
I
0.698
ENSPFOG00000002938
Poecilia formosa
ENSPFOG00000002938
I
0.698
ENSXMAG00000003651
Xiphophorus maculatus
ENSXMAG00000003651
I
0.698
TsM_000553100
Taenia solium
TsM_000553100
I
0.698
TTAC_0000027401
Hydatigera taeniaeformis
TTAC_0000027401
I
0.698
HNAJ_0001122601
Hymenolepis nana
HNAJ_0001122601
I
0.697
Smp_040350
Schistosoma mansoni
Smp_040350
I
0.696
A_01977
Schistosoma haematobium
A_01977
I
0.696
HDID_0000095701
Hymenolepis diminuta
HDID_0000095701
I
0.696
HmN_000672900
Hymenolepis microstoma
HmN_000672900
I
0.696
RFX7
Echinops telfairi
ENSETEG00000015037
I
0.695
NEMVEDRAFT_v1g25228
Nematostella vectensis
NEMVEDRAFT_v1g25228
I
0.694
CapteG131555
Capitella teleta
CapteG131555
I
0.693
rfx7
Tetraodon nigroviridis
ENSTNIG00000009322
I
0.693
rfx7
Takifugu rubripes
ENSTRUG00000017897
I
0.693
LotgiG138119
Lottia gigantea
LotgiG138119
I
0.689
Cmyd_10007628
Chelonia mydas
Cmyd_10007628
I
0.689
LDEC011897
Leptinotarsa decemlineata
LDEC011897
I
0.689
TC008959
Tribolium castaneum
TC008959
I
0.687
Sjc_0019090
Schistosoma japonicum
Sjc_0019090
I
0.687
SROB_0001335501
Schistosoma rodhaini
SROB_0001335501
I
0.687
DAPPUDRAFT_64123
Daphnia pulex
DAPPUDRAFT_64123
I
0.686
HMEL011744
Heliconius melpomene
HMEL011744
I
0.686
KGM_16732
Danaus plexippus
KGM_16732
I
0.685
MCINX003210
Melitaea cinxia
MCINX003210
I
0.685
PHUM035280
Pediculus humanus
PHUM035280
I
0.685
ENSAPLG00000002900
Anas platyrhynchos
ENSAPLG00000002900
I
0.684
ACEP24602
Atta cephalotes
ACEP24602
I
0.683
CGI_10006904
Crassostrea gigas
CGI_10006904
I
0.683
GB49419
Apis mellifera
GB49419
I
0.683
SINV24627
Solenopsis invicta
SINV24627
I
0.683
Hsal_12778--XP_001120516.1_APIME
Harpegnathos saltator
Hsal_12778--XP_001120516.1_APIME
I
0.683
NV23037
Nasonia vitripennis
NV23037
I
0.681
L798_03377
Zootermopsis nevadensis
L798_03377
I
0.680
CG9727
Drosophila melanogaster
FBgn0037445
I
0.678
GA21990
Drosophila pseudoobscura
FBgn0081975
I
0.678
GF16654
Drosophila ananassae
FBgn0093675
I
0.678
GG10435
Drosophila erecta
FBgn0102744
I
0.678
GH18082
Drosophila grimshawi
FBgn0125550
I
0.678
GI24583
Drosophila mojavensis
FBgn0147305
I
0.678
GL22063
Drosophila persimilis
FBgn0159655
I
0.678
GM10553
Drosophila sechellia
FBgn0165499
I
0.678
GD19544
Drosophila simulans
FBgn0191039
I
0.678
GJ22650
Drosophila virilis
FBgn0209754
I
0.678
GK13786
Drosophila willistoni
FBgn0215794
I
0.678
GE24099
Drosophila yakuba
FBgn0241238
I
0.678
LCUP014592
Lucilia cuprina
LCUP014592
I
0.678
MDOA009335
Musca domestica
MDOA009335
I
0.678
ADAC008404
Anopheles darlingi
ADAC008404
I
0.676
AGAP013226
Anopheles gambiae
AGAP013226
I
0.676
XP_002167798.1
Hydra magnipapillata
XP_002167798.1
I
0.676
AALB008384
Anopheles albimanus
AALB008384
I
0.676
AARA004226
Anopheles arabiensis
AARA004226
I
0.676
AATE016751
Anopheles atroparvus
AATE016751
I
0.676
ACHR008891
Anopheles christyi
ACHR008891
I
0.676
ACOM036649
Anopheles coluzzii
ACOM036649
I
0.676
ACUA017169
Anopheles culicifacies
ACUA017169
I
0.676
ADIR010232
Anopheles dirus
ADIR010232
I
0.676
AEPI006624
Anopheles epiroticus
AEPI006624
I
0.676
AFAF011654
Anopheles farauti
AFAF011654
I
0.676
AFUN011072
Anopheles funestus
AFUN011072
I
0.676
AMEC002251
Anopheles melas
AMEC002251
I
0.676
AMEM009524
Anopheles merus
AMEM009524
I
0.676
AMIN003942
Anopheles minimus
AMIN003942
I
0.676
AQUA006870
Anopheles quadriannulatus
AQUA006870
I
0.676
ASIS020285
Anopheles sinensis
ASIS020285
I
0.676
ASTEI04100
Anopheles stephensi
ASTEI04100
I
0.676
AAEL013752
Aedes aegypti
AAEL013752
I
0.675
CpipJ_CPIJ006094
Culex pipiens
CpipJ_CPIJ006094
I
0.674
BGIBMGA007758
Bombyx mori
BGIBMGA007758
I
0.674
CPIJ006094
Culex quinquefasciatus
CPIJ006094
I
0.674
CLEC011984
Cimex lectularius
CLEC011984
I
0.674
tetur02g00620
Tetranychus urticae
tetur02g00620
I
0.671
RFX5
Monodelphis domestica
ENSMODG00000018866
I
0.669
RFX5
Dasypus novemcinctus
ENSDNOG00000035113
I
0.668
Cflo_11342--XP_001120516.1_APIME
Camponotus floridanus
Cflo_11342--XP_001120516.1_APIME
I
0.668
ENSTSYG00000003230
Tarsius syrichta
ENSTSYG00000003230
I
0.667
RFX7
Ochotona princeps
ENSOPRG00000003155
I
0.663
ENSSSCG00000024042
Sus scrofa
ENSSSCG00000024042
I
0.663
Ogr11408
Ophisaurus gracilis
Ogr11408
I
0.663
ENSMEUG00000007846
Macropus eugenii
ENSMEUG00000007846
I
0.662
ISCW001129
Ixodes scapularis
ISCW001129
I
0.661
PTSG_12063
Salpingoeca rosetta
PTSG_12063
I
0.658
Cmyd_10014217
Chelonia mydas
Cmyd_10014217
I
0.658
D915_00887
Fasciola hepatica
D915_00887
I
0.658
SCUD_0001838101
Schistosoma curassoni
SCUD_0001838101
I
0.658
ECPE_0001841201
Echinostoma caproni
ECPE_0001841201
I
0.657
fgenesh2_pg.scaffold_3000303
Monosiga brevicollis
fgenesh2_pg.scaffold_3000303
I
0.655
CAOG_00356
Capsaspora owczarzaki
CAOG_00356
I
0.654
ACRE_029400
Acremonium chrysogenum
ACRE_029400
I
0.653
RFX7
Tupaia belangeri
ENSTBEG00000014637
I
0.653
ACYPI002124
Acyrthosiphon pisum
ACYPI002124
I
0.652
BDDG_02784
Blastomyces dermatitidis
BDDG_02784
I
0.651
CGI_10016120
Crassostrea gigas
CGI_10016120
I
0.651
estExt_fgenesh2_pg.C_390014
Monosiga brevicollis
estExt_fgenesh2_pg.C_390014
I
0.651
PV09_00065
Verruconis gallopava
PV09_00065
I
0.650
GPLIN_000191300
Globodera pallida
GPLIN_000191300
I
0.650