DRE_01490 (Drechslerella stenobrocha)
Zinc cluster

TF Information

Pfam ID Interpro ID Gene ID CIS-BP ID Sequence source Animal TF db
PF00172 (Zn_clus) IPR001138 DRE_01490 T366506_2.00 Ensembl (2018-Dec-8) Link out

Directly determined binding motifs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
No direct experiments

Motifs from related TFs

Name/Motif ID Species Forward Reverse Type/Study/Study ID SR
Score
DBD
Identity
FGRRES_09921
M02577_2.00
Fusarium graminearum
NNCGGVNNNN

NNNNBCCGNN
PBM
Weirauch et al.(2014)
pTH7794
0.597 0.575
acu-15
M02616_2.00
Neurospora crassa
NNCGGVNNNN

NNNNBCCGNN
PBM
Weirauch et al.(2014)
pTH7801
0.597 0.550
acu-15
M02617_2.00
Neurospora crassa
NNCGGVNNNN

NNNNBCCGNN
PBM
Weirauch et al.(2014)
pTH8900
0.597 0.550
ANIA_00689
M11469_2.00
Aspergillus nidulans Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
F$FACBALL_Q2
0.581 0.525
ANIA_00689
M11470_2.00
Aspergillus nidulans Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
F$FACBCA_Q2
0.581 0.525
ANIA_00689
M11471_2.00
Aspergillus nidulans Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
F$FACBCB_Q2
0.581 0.525
CAT8
M00102_2.00
Saccharomyces cerevisiae
NNNCCGGAN

NTCCGGNNN
PBM
Badis et al.(2008)
CAT8_4532
0.551 0.450
CAT8
M07529_2.00
Saccharomyces cerevisiae
CCGGRN

NYCCGG
PBM, CSA and or DIP-chip
Mathelier et al.(2014)
MA0280.1
0.551 0.450
CAT8
M08688_2.00
Saccharomyces cerevisiae
NCGGNNNDVNGGNN

NNCCNBHNNNCCGN
Misc
DeBoer et al.(2011)
YMR280C_33
0.551 0.450
CAT8
M11485_2.00
Saccharomyces cerevisiae Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
F$CAT8_Q6_01
0.551 0.450
CAT8
M11486_2.00
Saccharomyces cerevisiae Transfac license required
Transfac license required
Transfac
Matys et al.(2006)
F$CAT8_Q6
0.551 0.450
For this family, TFs with SR scores > 0.537 will likely have a similar motif

DNA Binding Domains

Protein ID Domain From To Sequence
EWC43603 Zinc cluster 58 97

Links

Other Zinc cluster family TFs
Other Drechslerella stenobrocha TFs

253 Related TFs

Name Species Gene ID Motif Evidence SR
Score
Action
AOL_s00080g113 Arthrobotrys oligospora AOL_s00080g113 I 0.682
H072_11612 Dactylellina haptotyla H072_11612 I 0.682
Bcin05g07910 Botrytis cinerea Bcin05g07910 I 0.621
W97_07671 Coniosporium apollinis W97_07671 I 0.621
BGHDH14_bgh03482 Blumeria graminis BGHDH14_bgh03482 I 0.620
MPH_09774 Macrophomina phaseolina MPH_09774 I 0.620
OIDMADRAFT_203117 Oidiodendron maius OIDMADRAFT_203117 I 0.620
SBOR_9682 Sclerotinia borealis SBOR_9682 I 0.620
UCRNP2_6692 Neofusicoccum parvum UCRNP2_6692 I 0.620
GMDG_07325 Pseudogymnoascus destructans GMDG_07325 I 0.612
V499_01314 Pseudogymnoascus pannorum V499_01314 I 0.612
CIMG_09602 Coccidioides immitis CIMG_09602 I 0.597
MCYG_02995 Microsporum canis MCYG_02995 I 0.597
VDBG_00686 Verticillium albo atrum VDBG_00686 I 0.597
ARB_01763 Arthroderma benhamiae ARB_01763 I 0.597
AUEXF2481DRAFT_25000 Aureobasidium subglaciale AUEXF2481DRAFT_25000 I 0.597
Bcin14g04080 Botrytis cinerea Bcin14g04080 I 0.597
BDDG_03989 Blastomyces dermatitidis BDDG_03989 I 0.597
CCM_00520 Cordyceps militaris CCM_00520 I 0.597
CFIO01_04476 Colletotrichum fioriniae CFIO01_04476 I 0.597
CGGC5_6484 Colletotrichum gloeosporioides CGGC5_6484 I 0.597
CHGG_00728 Chaetomium globosum CHGG_00728 I 0.597
CMQ_3248 Grosmannia clavigera CMQ_3248 I 0.597
Cob_09661 Colletotrichum orbiculare Cob_09661 I 0.597
CPC735_024080 Coccidioides posadasii CPC735_024080 I 0.597
CPUR_03154 Claviceps purpurea CPUR_03154 I 0.597
CSUB01_03093 Colletotrichum sublineola CSUB01_03093 I 0.597
CTHT_0052680 Chaetomium thermophilum CTHT_0052680 I 0.597
DOTSEDRAFT_67755 Dothistroma septosporum DOTSEDRAFT_67755 I 0.597
EURHEDRAFT_525504 Aspergillus ruber EURHEDRAFT_525504 I 0.597
F503_06102 Ophiostoma piceae uamh F503_06102 I 0.597
FFUJ_07541 Fusarium fujikuroi FFUJ_07541 I 0.597
FGRRES_09921 Fusarium graminearum FGRRES_09921 D 0.597
FOXG_05502 Fusarium oxysporum FOXG_05502 I 0.597
FPSE_03099 Fusarium pseudograminearum FPSE_03099 I 0.597
FVEG_02639 Fusarium verticillioides FVEG_02639 I 0.597
GLRG_10212 Colletotrichum graminicola GLRG_10212 I 0.597
GMDG_03942 Pseudogymnoascus destructans GMDG_03942 I 0.597
GSTUM_00005465001 Tuber melanosporum GSTUM_00005465001 I 0.597
H101_04897 Trichophyton interdigitale H101_04897 I 0.597
H102_08795 Trichophyton rubrum H102_08795 I 0.597
H105_08820 Trichophyton soudanense H105_08820 I 0.597
HCBG_02970 Histoplasma capsulatum HCBG_02970 I 0.597
HMPREF1624_05843 Sporothrix schenckii HMPREF1624_05843 I 0.597
M437DRAFT_48354 Aureobasidium melanogenum M437DRAFT_48354 I 0.597
M438DRAFT_266991 Aureobasidium pullulans M438DRAFT_266991 I 0.597
MAC_01154 Metarhizium acridum MAC_01154 I 0.597
MAJ_04331 Metarhizium majus MAJ_04331 I 0.597
MAM_07132 Metarhizium album MAM_07132 I 0.597
MANI_010830 Metarhizium anisopliae MANI_010830 I 0.597
MBM_00482 Marssonina brunnea MBM_00482 I 0.597
MBR_01591 Metarhizium brunneum MBR_01591 I 0.597
MCYG_02995 Arthroderma otae MCYG_02995 I 0.597
MGU_02269 Metarhizium guizhouense MGU_02269 I 0.597
MGYG_00169 Microsporum gypseum MGYG_00169 I 0.597
MPH_02048 Macrophomina phaseolina MPH_02048 I 0.597
MYCFIDRAFT_47796 Pseudocercospora fijiensis MYCFIDRAFT_47796 I 0.597
Mycgr3G66501 Zymoseptoria tritici Mycgr3G66501 I 0.597
MYCTH_2300537 Myceliophthora thermophila MYCTH_2300537 I 0.597
NechaG41465 Fusarium solani NechaG41465 I 0.597
NEUTE1DRAFT_127465 Neurospora tetrasperma NEUTE1DRAFT_127465 I 0.597
OCS_05092 Ophiocordyceps sinensis OCS_05092 I 0.597
OIDMADRAFT_40403 Oidiodendron maius OIDMADRAFT_40403 I 0.597
PAAG_11872 Paracoccidioides sp lutzii PAAG_11872 I 0.597
PABG_04235 Paracoccidioides brasiliensis PABG_04235 I 0.597
PCH_Pc13g12510 Penicillium rubens PCH_Pc13g12510 I 0.597
PDIP_26660 Penicillium digitatum PDIP_26660 I 0.597
PEX1_082400 Penicillium expansum PEX1_082400 I 0.597
PFICI_07248 Pestalotiopsis fici PFICI_07248 I 0.597
PITC_056580 Penicillium italicum PITC_056580 I 0.597
PODANS_2_2140 Podospora anserina PODANS_2_2140 I 0.597
PVAR5_8553 Byssochlamys spectabilis PVAR5_8553 I 0.597
S40285_06249 Stachybotrys chlorohalonata S40285_06249 I 0.597
S40288_04897 Stachybotrys chartarum S40288_04897 I 0.597
SEPMUDRAFT_146690 Sphaerulina musiva SEPMUDRAFT_146690 I 0.597
SMAC_06552 Sordaria macrospora SMAC_06552 I 0.597
SPBR_00143 Sporothrix brasiliensis SPBR_00143 I 0.597
SS1G_10339 Sclerotinia sclerotiorum SS1G_10339 I 0.597
TEQG_08271 Trichophyton equinum TEQG_08271 I 0.597
TESG_07986 Trichophyton tonsurans TESG_07986 I 0.597
THITE_2106669 Thielavia terrestris THITE_2106669 I 0.597
TRIATDRAFT_221379 Trichoderma atroviride TRIATDRAFT_221379 I 0.597
TRIREDRAFT_49232 Trichoderma reesei TRIREDRAFT_49232 I 0.597
TRIVIDRAFT_166933 Trichoderma virens TRIVIDRAFT_166933 I 0.597
TRV_01331 Trichophyton verrucosum TRV_01331 I 0.597
U727_0043367091 Penicillium solitum U727_0043367091 I 0.597
UCRNP2_919 Neofusicoccum parvum UCRNP2_919 I 0.597
UCRPA7_1587 Togninia minima UCRPA7_1587 I 0.597
UREG_04565 Uncinocarpus reesii UREG_04565 I 0.597
UV8b_3025 Ustilaginoidea virens UV8b_3025 I 0.597
V499_01421 Pseudogymnoascus pannorum V499_01421 I 0.597
VDAG_00301 Verticillium dahliae VDAG_00301 I 0.597
VDBG_00686 Verticillium alfalfae VDBG_00686 I 0.597
VHEMI01993 Torrubiella hemipterigena VHEMI01993 I 0.597
X797_002104 Metarhizium robertsii X797_002104 I 0.597
e_gw1.5.1078.1 Nectria haematococca e_gw1.5.1078.1 I 0.597
estExt_fgenesh2_pg.C_10678 Neurospora discreta estExt_fgenesh2_pg.C_10678 I 0.597
estExt_Genewise1.C_10162 Mycosphaerella fijiensis estExt_Genewise1.C_10162 I 0.597
estExt_Genewise1Plus.C_chr_12372 Mycosphaerella graminicola estExt_Genewise1Plus.C_chr_12372 I 0.597
acu-15 Neurospora crassa NCU06656 D 0.597
Pc13g12510 Penicillium chrysogenum Pc13g12510 I 0.597
GSTUM_00004762001 Tuber melanosporum GSTUM_00004762001 I 0.596
SNOG_10771 Stagonospora nodorum SNOG_10771 I 0.581
A1O1_06777 Capronia coronata A1O1_06777 I 0.581
A1O3_10373 Capronia epimyces A1O3_10373 I 0.581
A1O5_07453 Cladophialophora psammophila A1O5_07453 I 0.581
A1O7_09708 Cladophialophora yegresii A1O7_09708 I 0.581
A1O9_05407 Exophiala aquamarina A1O9_05407 I 0.581
ACRE_084730 Acremonium chrysogenum ACRE_084730 I 0.581
BAUCODRAFT_120474 Baudoinia compniacensis BAUCODRAFT_120474 I 0.581
ACLA_021920 Aspergillus clavatus CADACLAG00001871 I 0.581
CADAFLAG00003675 Aspergillus flavus CADAFLAG00003675 I 0.581
AFUA_1G13510 Aspergillus fumigatus CADAFUAG00007202 I 0.581
CADANGAG00006980 Aspergillus niger CADANGAG00006980 I 0.581
ANIA_00689 Aspergillus nidulans CADANIAG00001981 D 0.581
CADAORAG00006095 Aspergillus oryzae CADAORAG00006095 I 0.581
ATEG_00558 Aspergillus terreus CADATEAG00006881 I 0.581
NFIA_011990 Neosartorya fischeri CADNFIAG00001575 I 0.581
COCC4DRAFT_160202 Bipolaris maydis COCC4DRAFT_160202 I 0.581
COCCADRAFT_3273 Bipolaris zeicola COCCADRAFT_3273 I 0.581
COCMIDRAFT_23407 Bipolaris oryzae COCMIDRAFT_23407 I 0.581
COCSADRAFT_243527 Bipolaris sorokiniana COCSADRAFT_243527 I 0.581
COCVIDRAFT_103972 Bipolaris victoriae COCVIDRAFT_103972 I 0.581
G647_08575 Cladophialophora carrionii G647_08575 I 0.581
GGTG_09932 Gaeumannomyces graminis GGTG_09932 I 0.581
HMPREF1120_01646 Exophiala dermatitidis HMPREF1120_01646 I 0.581
HMPREF1541_02579 Cyphellophora europaea HMPREF1541_02579 I 0.581
LEMA_P079800.1 Leptosphaeria maculans LEMA_P079800.1 I 0.581
MAPG_02877 Magnaporthe poae MAPG_02877 I 0.581
MGG_04108 Magnaporthe oryzae MGG_04108 I 0.581
PDE_00198 Penicillium oxalicum PDE_00198 I 0.581
PMAA_067280 Talaromyces marneffei PMAA_067280 I 0.581
PTRG_09927 Pyrenophora triticirepentis PTRG_09927 I 0.581
PTT_11750 Pyrenophora teres PTT_11750 I 0.581
PV04_10755 Capronia semiimmersa PV04_10755 I 0.581
PV05_02803 Exophiala xenobiotica PV05_02803 I 0.581
PV06_08403 Exophiala oligosperma PV06_08403 I 0.581
PV07_12426 Cladophialophora immunda PV07_12426 I 0.581
PV08_06719 Exophiala spinifera PV08_06719 I 0.581
PV09_05217 Verruconis gallopava PV09_05217 I 0.581
PV10_01410 Exophiala mesophila PV10_01410 I 0.581
PV11_05983 Exophiala sideris PV11_05983 I 0.581
SETTUDRAFT_182790 Setosphaeria turcica SETTUDRAFT_182790 I 0.581
SNOG_10771 Phaeosphaeria nodorum SNOG_10771 I 0.581
TSTA_087380 Talaromyces stipitatus TSTA_087380 I 0.581
UCREL1_9979 Eutypa lata UCREL1_9979 I 0.581
W97_03320 Coniosporium apollinis W97_03320 I 0.581
Z517_12110 Fonsecaea pedrosoi Z517_12110 I 0.581
Z518_06304 Rhinocladiella mackenziei Z518_06304 I 0.581
Z519_06020 Cladophialophora bantiana Z519_06020 I 0.581
estExt_Genewise1Plus.C_11533 Cochliobolus heterostrophus C5 estExt_Genewise1Plus.C_11533 I 0.581
AB00291.1 Alternaria brassicicola AB00291.1 I 0.581
estExt_fgenesh1_pm.C_60502 Cryphonectria parasitica estExt_fgenesh1_pm.C_60502 I 0.581
fgenesh1_pm.00083_#_404 Aspergillus carbonarius fgenesh1_pm.00083_#_404 I 0.581
PMAA_067280 Penicillium marneffei PMAA_067280 I 0.581
BN7_5956 Wickerhamomyces ciferrii BN7_5956 I 0.579
ZBAI_00324 Zygosaccharomyces bailii ZBAI_00324 I 0.579
ZYRO0G14278g Zygosaccharomyces rouxii ZYRO0G14278g I 0.579
SNOG_09792 Stagonospora nodorum SNOG_09792 I 0.573
COCC4DRAFT_156048 Bipolaris maydis COCC4DRAFT_156048 I 0.573
COCCADRAFT_94279 Bipolaris zeicola COCCADRAFT_94279 I 0.573
COCMIDRAFT_80931 Bipolaris oryzae COCMIDRAFT_80931 I 0.573
COCSADRAFT_166678 Bipolaris sorokiniana COCSADRAFT_166678 I 0.573
COCVIDRAFT_83730 Bipolaris victoriae COCVIDRAFT_83730 I 0.573
LEMA_P068880.1 Leptosphaeria maculans LEMA_P068880.1 I 0.573
PTRG_10684 Pyrenophora triticirepentis PTRG_10684 I 0.573
PTT_14070 Pyrenophora teres PTT_14070 I 0.573
SETTUDRAFT_41285 Setosphaeria turcica SETTUDRAFT_41285 I 0.573
SNOG_09792 Phaeosphaeria nodorum SNOG_09792 I 0.573
TBLA_0D05420 Tetrapisispora blattae TBLA_0D05420 I 0.573
e_gw1.2.33.1 Cochliobolus heterostrophus C5 e_gw1.2.33.1 I 0.573
AB00158.1 Alternaria brassicicola AB00158.1 I 0.573
YALI0_C19151g Yarrowia lipolytica YALI0_C19151g I 0.572
AOL_s00173g90 Arthrobotrys oligospora AOL_s00173g90 I 0.568
DRE_06992 Drechslerella stenobrocha DRE_06992 I 0.568
CMQ_423 Grosmannia clavigera CMQ_423 I 0.566
HMPREF1624_08770 Sporothrix schenckii HMPREF1624_08770 I 0.566
SAPIO_CDS1259 Scedosporium apiospermum SAPIO_CDS1259 I 0.566
SPBR_02251 Sporothrix brasiliensis SPBR_02251 I 0.566
BAUCODRAFT_101870 Baudoinia compniacensis BAUCODRAFT_101870 N 0.563
DOTSEDRAFT_70721 Dothistroma septosporum DOTSEDRAFT_70721 N 0.562
MYCFIDRAFT_137203 Pseudocercospora fijiensis MYCFIDRAFT_137203 N 0.562
Mycgr3G21814 Zymoseptoria tritici Mycgr3G21814 N 0.562
SEPMUDRAFT_148074 Sphaerulina musiva SEPMUDRAFT_148074 N 0.562
estExt_Genewise1Plus.C_410011 Mycosphaerella fijiensis estExt_Genewise1Plus.C_410011 N 0.562
gw1.1.2293.1 Mycosphaerella graminicola gw1.1.2293.1 N 0.562
CPAG_00982 Candida parapsilosis CPAG_00982 I 0.555
CORT_0B04760 Candida orthopsilosis CORT_0B04760 I 0.555
GNLVRS01_PISO0K20118g Millerozyma farinosa GNLVRS01_PISO0K20118g I 0.555
GNLVRS01_PISO0L20119g Millerozyma farinosa GNLVRS01_PISO0L20119g I 0.555
KLLA0_D01452g Kluyveromyces lactis KLLA0_D01452g I 0.555
LELG_01997 Lodderomyces elongisporus LELG_01997 I 0.555
PICST_31755 Scheffersomyces stipitis PICST_31755 I 0.555
SPAPADRAFT_154694 Spathaspora passalidarum SPAPADRAFT_154694 I 0.555
fgenesh1_pg.C_chr_4.1000626 Pichia stipitis fgenesh1_pg.C_chr_4.1000626 I 0.555
VDBG_09923 Verticillium albo atrum VDBG_09923 N 0.554
VDBG_09923 Verticillium alfalfae VDBG_09923 N 0.554
AACERI_AaceriABL121C Saccharomycetaceae sp ashbya aceri AACERI_AaceriABL121C I 0.551
AGOS_ABL121C Ashbya gossypii AGOS_ABL121C I 0.551
CAGL0M03025g Candida glabrata CAGL0M03025g I 0.551
Ecym_4616 Eremothecium cymbalariae Ecym_4616 I 0.551
KAFR_0B03950 Kazachstania africana KAFR_0B03950 I 0.551
KLTH0C03762g Lachancea thermotolerans KLTH0C03762g I 0.551
LALA0_S03e00276g Lachancea lanzarotensis LALA0_S03e00276g I 0.551
NCAS_0C00390 Naumovozyma castellii NCAS_0C00390 I 0.551
NDAI_0K00390 Naumovozyma dairenensis NDAI_0K00390 I 0.551
PAS_chr2-1_0757 Komagataella pastoris PAS_chr2-1_0757 I 0.551
PICST_622 Scheffersomyces stipitis PICST_622 I 0.551
SKUD_207003 Saccharomyces kudriavzevii SKUD_207003 I 0.551
TDEL_0B00530 Torulaspora delbrueckii TDEL_0B00530 I 0.551
CAT8 Saccharomyces cerevisiae YMR280C D 0.551
gwh1.2.1.110.1 Pichia stipitis gwh1.2.1.110.1 I 0.551
Scas_Contig696.44 Saccharomyces castellii Scas_Contig696.44 I 0.551
17487_YMR280C Saccharomyces mikatae 17487_YMR280C I 0.551
17891_YMR280C Saccharomyces paradoxus 17891_YMR280C I 0.551
19463_YMR280C Saccharomyces bayanus 19463_YMR280C I 0.551
KLTH0C03762g Kluyveromyces thermotolerans KLTH0C03762g I 0.551
Kwal_10232 Kluyveromyces waltii Kwal_10232 I 0.551
SAKL0D01342g Lachancea kluyveri SAKL0D01342g I 0.551
XP_002491690.1 Pichia pastoris XP_002491690.1 I 0.551
KNAG_0B01840 Kazachstania naganishii KNAG_0B01840 I 0.549
LALA0_S08e06744g Lachancea lanzarotensis LALA0_S08e06744g I 0.549
AUEXF2481DRAFT_639986 Aureobasidium subglaciale AUEXF2481DRAFT_639986 N 0.545
M437DRAFT_41048 Aureobasidium melanogenum M437DRAFT_41048 N 0.545
M438DRAFT_358845 Aureobasidium pullulans M438DRAFT_358845 N 0.545
CHGG_02115 Chaetomium globosum CHGG_02115 N 0.544
UV8b_6675 Ustilaginoidea virens UV8b_6675 N 0.544
CPUR_07537 Claviceps purpurea CPUR_07537 N 0.543
CSUB01_01308 Colletotrichum sublineola CSUB01_01308 N 0.543
CTHT_0074660 Chaetomium thermophilum CTHT_0074660 N 0.543
F503_07581 Ophiostoma piceae uamh F503_07581 N 0.543
FFUJ_03889 Fusarium fujikuroi FFUJ_03889 N 0.543
FGRRES_04888_M Fusarium graminearum FGRRES_04888_M N 0.543
FOXG_05736 Fusarium oxysporum FOXG_05736 N 0.543
FPSE_03993 Fusarium pseudograminearum FPSE_03993 N 0.543
FVEG_03609 Fusarium verticillioides FVEG_03609 N 0.543
GLRG_08586 Colletotrichum graminicola GLRG_08586 N 0.543
MYCTH_2307849 Myceliophthora thermophila MYCTH_2307849 I 0.543
NechaG49659 Fusarium solani NechaG49659 N 0.543
PFICI_02612 Pestalotiopsis fici PFICI_02612 N 0.543
THITE_2120534 Thielavia terrestris THITE_2120534 I 0.543
VHEMI09842 Torrubiella hemipterigena VHEMI09842 N 0.543
e_gw1.43.151.1 Nectria haematococca e_gw1.43.151.1 N 0.543
e_gw1.4.1073.1 Cryphonectria parasitica e_gw1.4.1073.1 N 0.543
CAGL0L03377g Candida glabrata CAGL0L03377g I 0.542
GNLVRS01_PISO0I17586g Millerozyma farinosa GNLVRS01_PISO0I17586g I 0.542
GNLVRS01_PISO0J19347g Millerozyma farinosa GNLVRS01_PISO0J19347g I 0.542
KAFR_0A01480 Kazachstania africana KAFR_0A01480 I 0.542
KLLA0_F14322g Kluyveromyces lactis KLLA0_F14322g I 0.542
Kpol_1016p20 Vanderwaltozyma polyspora Kpol_1016p20 I 0.542
ZBAI_02613 Zygosaccharomyces bailii ZBAI_02613 I 0.542
ZBAI_07009 Zygosaccharomyces bailii ZBAI_07009 I 0.542
ZYRO0G15136g Zygosaccharomyces rouxii ZYRO0G15136g I 0.542